Acid phosphatase is synthesized in the biosynthetic pathway in the ER and packaged in the TGN into primary lysosomes which are passed on to secondary lysosomes. Primary lysosomes are small vesicles of 75-100nm filled with acid phosphatase and no substrate. They probably fuse with secondary lysosomes before the secondary lysosomes fuse with the DV-II. Small vesicles of the size of primary lysosomes are near secondary lysosomes. TEM taken on 3/26/79 by J. Muraoka with Hitachi HU11A operating at 75kV. Neg. 25,500X. Bar = 0.2µm.
Glutaraldehyde fixation followed by Gomori Staining, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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