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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36812*  Cite 

Isolated microtubules and kinesin spontaneously self-organize in vitro creating assemblies that resemble bundles of cilia that undergo synchronous beating. The major components are fluorescent taxol-stabilized tubulin, biotin-kinesin, and streptavidin. The movie shows tubulin trapped at the air-glass interface of an air bubble that has been confined in a 10-μm-thick chamber. The microtubules nucleate assemblies of active bundles perpendicular to the edge of the bubble, and provide important insights into the mechanism of synchronized ciliary beating observed in vivo.

Technical Details

The movie was recorded using Nikon Eclipse Ti epifluorescence microscope equipped with an Andor Clara CCD camera. See T. Sanchez et al. 2011. Cilia-like beating of active microtubule bundles. Science 333:456-459.

Biological Sources
Cellular Component
Biological Context
Biological Process
cilium movement
Timothy Sanchez
David Welch
Daniela Nicastro
Zvonimir Dogic
NIGMS BioMedical Beat
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
intensified CCD (ICCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Alexa Fluor 647
Data Qualifiers
processed data
Sample Preparation
in vitro assembly
Spatial Axis Image Size Pixel Size
X 234px ——
Y 234px ——