Electron microscope autoradiograph showing labeled material in a pancreatic duct 4 hours post injection. Image corresponds to Figure 12 in Caro, L. G., and G. E. Palade. 1964 J. Cell Biol. 20:473-495. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
Anesthetized animals were intravenously injected with 1-5 millicuris of DL-leucine 4,5-H^3 with a specific activity of 3570 mc/mM. At times ranging from 4 min to 15 hrs after injection the pancreas was removed and fixed in osmium tetroxide. Tissue was embedded in methacrylate, sectioned, and post-stained with uranyl acetate. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi.
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