NCBI Organism:Rattus; Cell Types:CNS neuron (sensu Vertebrata) Cell Components:dendritic spine, dendrite, dendritic shaft, cytoplasm; Biological process:dendritic spine development, dendritic spine morphogenesis;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37157^* Cite

Description

This is Video S2 which corresponds to still images in top row of Figure 2D. It shows the spine dynamics of control neurons. Time-lapse confocal imaging was performed on DIV14 hippocampal neurons co-expressing GFP. Images were acquired every 1-minute using confocal microscopy. 3 frames/sec shown.

Technical Details

Confocal images were collected on an Olympus Fluoview 1000 microscope (IX81 base) equipped with a 60X/1.35 NA (oil)UPLSAPO 60X objective (Olympus). GFP was excited using the 488 nm laser line of a multi Ar laser. Fluorescence emission was collected using the following dichroic mirror/filter: SDM560/BA505–525 (GFP).

Biological Sources

NCBI Organism Classification: Rattus
Cell Type: CNS neuron (sensu Vertebrata)
Cellular Component: dendritic spine; dendrite; dendritic shaft; cytoplasm

Biological Context

Biological Process: dendritic spine development; dendritic spine morphogenesis
Molecular Function: myosin II light chain binding; ATPase activity

Attribution

Names: Jennifer L. Hodges; Karen Newell-Litwa; Hannelore Asmussen; Miguel Vicente-Manzanares; Alan Rick Horwitz
Link: http://www.plosone.org/article/info%3Adoi%2F1...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL37157
Archival Resource Key (ARK): ark:/b7295/w9cil37157

Grouping This image is part of a group.

Imaging

Image Type: time lapse microscopy
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	759px	0.06µm
Y	367px	0.06µm

Time	60 seconds	20