This is Video S2 which corresponds to still images in top row of Figure 2D. It shows the spine dynamics of control neurons. Time-lapse confocal imaging was performed on DIV14 hippocampal neurons co-expressing GFP. Images were acquired every 1-minute using confocal microscopy. 3 frames/sec shown.
Confocal images were collected on an Olympus Fluoview 1000 microscope (IX81 base) equipped with a 60X/1.35 NA (oil)UPLSAPO 60X objective (Olympus). GFP was excited using the 488 nm laser line of a multi Ar laser. Fluorescence emission was collected using the following dichroic mirror/filter: SDM560/BA505–525 (GFP).
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