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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37200*  Cite 
Description

Transmission electron micrograph of guinea pig pancreas mitochondria and lipid droplets. The use of osmium tetroxide as a fixative for electron microscopy was first described by Dr. Palade at the Rockefeller. Another major advance in fixation for electron microscopy was done at Yale by David Sabatini who described the use of glutaraldehyde which not only preserved ultrastructure, but enzymatic activities which opened up the possibility of EM localization of enzymatic function in cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

References: Palade, G.E. 1952. J. Exp. Med. 95:285-298. Palade, G.E. 1952. Anat. Rec. 114:427-451. Sabatini, D.D, K. Bensch and R.J. Barrnett. 1963. J. Cell. Bio. 17:19-58. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X12,100.

Biological Sources
NCBI Organism Classification
Cavia porcellus
Cell Type
pancreas
Cellular Component
mitochondrion
lipid droplets
mitochondrial crista
Attribution
Names
George E. Palade
Link
George E. Palade EM Slide Collection
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL37200
Archival Resource Key (ARK)
ark:/b7295/w9cil37200
Grouping This image is part of a group.
Sample Preparation
Methods
osmium tetroxide fixed tissue
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 6000px ——
Y 4727px ——