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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37299*  Cite 

Transmission electron micrograph of rough microsomes obtained by fraction of guinea pig pancreas. Cell fractionation confirmed that newly synthesized acinar cell secretory proteins moved from the rough endoplasmic reticulum to the periphery of the Golgi in the lumen of a smooth-surfaced membrane bounded compartment. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Additional technical details regarding cell fractionation, specimen processing, and electron microscopy can be found in: Jamieson, J.D., and Palade, G.E. J. Cell Biol. 34:577-596, 1967. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x10,000

Biological Sources
NCBI Organism Classification
Cavia porcellus
Cell Type
pancreatic cell
Cellular Component
rough microsome
Biological Context
Biological Process
protein secretion
James D. Jamieson
George E. Palade EM Slide COllection
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
lead salt
uranyl salt
Processing History
scanned negative
Sample Preparation
osmium tetroxide fixed tissue
Relation To Intact Cell
isolated subcellular component
Spatial Axis Image Size Pixel Size
X 6000px ——
Y 4530px ——