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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37338*  Cite 

Promyelocytic leukemia nuclear bodies (PML NBs) are dynamic nuclear organelles. They range from 0.1um to 1um in size and exist in most of mammalian cell types. The functions of PML NBs are indicated by their association with a variety of component proteins that are involved in different cellular process. Here shows PML NBs labeled by monoclonal antibody (PML36) in 3T3 cells. .

Technical Details

Cells were rinsed in PBS once and fixed for 15 min in 2% formaldehyde in PBS, pH 7.4. After being washed in PBS for 10 min x3, cells were permeablized 5 min in PBS with 0.2% Triton X-100 and 1% normal goat serum. After being washed in PBS plus 1% goat serum for 10 min x3, the following primary antibody (PML36) were added for 1 h at RT. Cells were rinsed in PBS with 1% normal goat serum for 10 min x3, and secondary FITC anti-mouse antibodies were added for 1 h at RT. Cells were rinsed in PBS for 10 min x3 and were observed on a modified Zeiss Axiovert 200M microscope with a plan-neofluar ×40 objective. The microscope was fitted with dual excitation and emission filter wheels and a Photometrics Coolsnap-HQ camera.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cell Line
Cellular Component
PML body
Yi-Chun Maria Chen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
fluorescence microscopy
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
distribution of epitope
Visualization Methods
Fluorescein (FITC)
primary antibody plus labeled secondary antibody
Processing History
maximum projection
Data Qualifiers
processed data
Sample Preparation
detergent permeabilized
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 292px 0.16µm
Y 240px 0.16µm