This photoactivation localization microscopy (PALM) image of tdEos-paxillin (green) and PsCFP20-zyxin (red) demonstrates that these two focal adhesion proteins are have very little over-lap when visualized with super-resolution. In contrast, when the same field is visualized with total internal reflection (TIRF) microscopy, the two proteins appear almost completely colocalized CIL 38604. Note the differential interference contrast (DIC) image corresponding to the same image field is also available as CIL 38603. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 4 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
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