Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. Under the conditions used here, with the ac-31 mutant, thylakoids are completely unstacked. See Fig 19 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.
Isolated chloroplast membranes were fixed with glutaraldehyde, post fixed with osmium tetroxide, dehydrated, embedded in epoxy resin, and thin sections cut, stained and examined with a Hitachi HU-11C TEM.
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