Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. With the ac-31 mutant, and the in vitro conditions used, thylakoid membranes form stacked regions with fracture faces showing the characteristic particle sizes and distributions. See Fig 20 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.
Idolated thylakoid membranes were cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619
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