Dorsal root ganglion cells from embryonic mice were cultured and stimulated to produce cyclooxygenase-2 (COX-2). Cultures were immuno-stained for neuron-associated tubulin (green), COX-2 (red) and DNA (blue). COX-2 is primarily expressed in neurons.
After induction of COX-2, cultures were fixed with paraformaldehyde overnight, then immunostained with a mouse antibody to neuronal class III beta-tubulin followed by an Alexa 488-conjugated goat anti-mouse to identify neurons. COX-2 was imaged using Fluorocoxib A, a selected COX-2 inhibitor conjugated to carboxy-X-rhodamine (see http://cancerres.aacrjournals.org/content/70/9/3618.full). Preparations were counterstained with DAPI, and imaged with a Leica CMS microscope using a 40x 0.8 NA objective lens. Images were recorded with a Qimaging Qi CCD camera.
|Spatial Axis||Image Size||Pixel Size|