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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38870*  Cite 
Description

The cytosol contains numerous vacuoles as well as symbiotic bacteria (see Heckmann, Int. Rev. Cytol. 14(suppl.):111-143, 1983 for a discussion of symbiotic bacteria in Euplotes). TEM taken on 7/24/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Euplotes sp.
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Euplotes sp. from Carolina Biological Supply
Cellular Component
cytoplasm
symbiont-containing vacuole
cytoplasmic symbiont
Biological Context
Biological Process
cytoplasm organization
detection of symbiont
endosymbiont
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38870
Archival Resource Key (ARK)
ark:/b7295/w9cil38870
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 2998px 1.4nm
Y 4000px 1.4nm