Wheat seeds were germinated and grown for 6 days in the dark, greened at high light intensity for 4 days, thylakoids prepared, plunge-frozen, and freeze-fracture replicas obtained and examined by transmission electron microscopy. This region from a wild type plant shows the characteristic particle size and distribution found on the EF and PF membrane faces, with the different distributions between stacked (PFs and EFs) and unstacked (PFu, EFu)thylakoid regions. This set of grouped images, which also shows thin sections, includes images of the chlorophyll b-deficient CD3 mutant.