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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39126*  Cite 
Description

Thin section of a chloroplast of Arabidopsis thaliana lacking the FZL protein, a member of the dynamin superfamily of proteins. FZL is related to FZO, a protein that mediates fusion between mitochondrial outer membrages in animals and fungi. The characteristic distribution of thylakoids membranes in stacked grana (black arrow) and unstacked stromal regions (white arrows) was disorganized with grana lamellae less uniform in length and stacked in a staggered fashion. Asterisks denote vesicle-like structures that occur in the absence of FZL. The other image in this group (CIL 39125) shows the wildtype structure.

Technical Details

3-week old leaf tissue from Arabidopsis thaliana (eco-type Columbia - Col-0) was fixed, embedded in resin, and 70 nm serial sections cut, stained, and observed by transmission EM. See Fig 2 in H Gao et al, 2006. Proc Natl Acad Sci USA 103:6759-6764.

Biological Sources
NCBI Organism Classification
Arabidopsis thaliana
Cell Type
leaf
Cellular Component
chloroplast
plastid thylakoid
granum
Biological Context
Biological Process
C4 photosynthesis
organelle organization
Attribution
Names
Hongbo Gao
Tammy L Sage
Katherine W Osteryoung
Published
H Gao, TL Sage, KW Osteryoung 2006 FZL, an FZO-like protein in plants is a determinant of thylakoid and chloroplast morphology. Proc Natl Acad Sci USA 103:6759-6764
Pubmed
21646186
Link
Proceedings National Academy of Science USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39126
Archival Resource Key (ARK)
ark:/b7295/w9cil39126
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Data Qualifiers
processed data
Sample Preparation
Methods
glutaraldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 250px ——
X 184px ——