Dictyosomal elements of the Golgi complex consist of a layer of coated transition vesicles next to a specialized area of ER called the transition zone and two or three highly fenestrated layers of cisternae next to this. Clathrin-coated vesicles arise from the mature face of a dictyosomal-element, also called the trans Golgi network. TEM taken 10/30/68 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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