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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39202*  Cite 
Description

Single microtubules extend into the cytoplasm from the tips of the cytoproct ridge. These are thought to come to lie along the surface of the approaching DV-IV and to pull the ridge down against the DV-IV and to permit the plasma membrane at the crest of the ridge to fuse with the tubular extensions of the DV-IV. (Published and discussed in Allen and Wolf, J. Cell Sci. 14:611-631, 1974). TEM taken on 6/1/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,500X. Adapted with permission.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cytoproct
vesicle membrane
microtubule associated complex
Biological Context
Biological Process
digestive system process
egestion
Attribution
Names
Richard Allen (University of Hawaii)
Published
J Cell Sci. 1974 May;14(3):611-31.
Published
4364579
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39202
Archival Resource Key (ARK)
ark:/b7295/w9cil39202
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4707px 0.81nm
Y 5247px 0.81nm