Single microtubules extend into the cytoplasm from the tips of the cytoproct ridge. These are thought to come to lie along the surface of the approaching DV-IV and to pull the ridge down against the DV-IV and to permit the plasma membrane at the crest of the ridge to fuse with the tubular extensions of the DV-IV. (Published and discussed in Allen and Wolf, J. Cell Sci. 14:611-631, 1974). TEM taken on 6/1/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,500X. Adapted with permission.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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