Mouse myoblasts stably transfected with GFP-PCNA to label replication foci and the 3D distribution of the GFP signal recorded over time using a confocal microscope. The time series illustrates the dynamic changes in relative strength of the GFP signal in individual replication factories.
Living C2C12 cells stably expressing GFP-PCNA were observed with a Leica TCS confocal microscope and z-series collected at 0.5 micrometer intervals over a 90 min period. See: Fig 6B in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
|Spatial Axis||Image Size||Pixel Size|