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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39513*  Cite 

A thin cryosection of Vorticella exposed to a rabbit polyclonal antibody to alpha-centrin and then to gold conjugated to anti-rabbit IgG. The gold indicates that the myoneme contains alpha-centrin. The plasma membrane, outer alveolar membrane and inner alveolar membrane all appear as transparent tracks but the lumen of the alveolus appears electron opaque. Thickenings of the ER lying against the myoneme correspond to linkage complexes. TEM taken on 8/18/94 by D. Kunkel with Zeiss 10A operating at 80kV. Neg. 19,800X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (

Technical Details

Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73.

Biological Sources
NCBI Organism Classification
Vorticella convallaria
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
plasma membrane
Biological Context
Biological Process
regulation of myofibril size
plasma membrane organization
centrin localization
Molecular Function
centrin localization
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
Processing History
recorded image
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 4210px 1nm
Y 3616px 1nm