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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39686*  Cite 

Intestinal epithelium from the adult midgut of Drosophila melanogaster were immuno-stained for the cell periphery (integrin, red), nuclei (DAPI, blue) and stem cells (green). Nuclei are not seen in the tiny stem cells. The system was used to determine the role of intestinal stem cells in establishing the size of the gut in response to changes in food availability.

Technical Details

Midguts were fixed with 8% formaldehyde for 20 min at room temperature, and immunolabeled with primary antibodies bPS integrin (cf.6g11) and disc large (4F3), and stained with DAPI. The tissue was examined using a Leica TCs confocal microscope with a 63x N.A. 1.4 objective lens. Images were contrast enhanced and stem cells pseudocolored. See also: L E O'Brien et al., (2011) Altered modes of stem cell division drive adaptive intestinal growth. Cell 147:603-614.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
intestinal epithelial cell
stem cell
Cellular Component
cell membrane
Biological Context
Biological Process
regulaton of gut size
Lucy O'Brien
David Bilder
NIGMS Biomedical Beat
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
4',6-diamidino-2-phenylindole (DAPI)
Alexa Fluor 555
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 400px ——
Y 307px ——