Transmission electron micrograph showing the nucleus of a mouse cardiac tissue with nucleolus. At the center of the image is a large structure called the nucleus which is a membrane-limited compartment within the cell that contains the genetic material, DNA, in the form of chromosomes. Within the nucleus is the nucleolus is a small, darkly staining mass within the nucleus wherein ribonucleoprotein is produced. Within the nucleolus it is easy to see the four small circular areas called the nucleolar organizing region is initially where RNA and its associated proteins are processed.
Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.
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