Tomographic reconstruction of a dendrite and dendritic spines from a Purkinje neuron in the rat cerebellum. This image is a maximum intensity projection through the volume of a segment of dendrite in a 2 um section, with contrast reversed so that dendrite appears bright against background. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
The tomogram was generated using an JEOL4000 IVEM. Single tilt images spanned -60 to 60° in 2° increments. Magnification, 3000X; accelerating voltage, 400.0 KeV.
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