Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39949^* Cite

Description

Tomographic reconstruction of a dendrite and dendritic spines from a Purkinje neuron in the rat cerebellum. This image is a maximum intensity projection through the volume of a segment of dendrite in a 2 um section, with contrast reversed so that dendrite appears bright against background. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.

Technical Details

The tomogram was generated using an JEOL4000 IVEM. Single tilt images spanned -60 to 60° in 2° increments. Magnification, 3000X; accelerating voltage, 400.0 KeV.

Biological Sources

NCBI Organism Classification: Rattus norvegicus
Cell Type: CNS neuron (sensu Vertebrata); Purkinje cell
Cellular Component: dendritic spine; dendrite

Attribution

Names: Maryann Martone; Naoko Yamada; Gordun Arbuthnott; Cali Ingham; Stephen Young
Link: CCDB 13

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39949
Archival Resource Key (ARK): ark:/b7295/w9cil39949

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM); detection of electrons; illumination by electrons
Parameters Imaged: electron density
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	171px	——
Y	583px	——