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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40005*  Cite 

Single computed slice from a tomographic reconstruction of a 500 nm section through the middle free wall of a cardiac myocyte from the left ventricle of an adult mouse (6-8 months of age), stained with potassium ferrocyanide to increase the contrast of membrane systems. For more information see: Hayashi, T., Martone, M. E., Yu, Z., Thor, A., Doi, M., Holst, M., Ellisman, M. H. and Hoshijima, M., Three-dimensional electron microscopy reveals new details of membrane systems for calcium signaling in the heart. J. Cell Science, 2009 Apr 1;122(Pt 7):1005-13. PMID: 19295127. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Tissue was fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.15M sodium cacodylate, blocked into small pieces, post-fixed for 1-3 hours, washed 3x10min in 0.15M sodium cacodylate, then incubated in: 0.8% potassium ferrocyanide and 2% osmium tetroxide in 0.15M sodium cacodylate overnight at room temperature. Following another set of washes 2 hours-overnight in 0.15M sodium cacodylate tissue was stained en bloc with 1% uranyl acetate 30min, followed by a brief wash in DDH2O and standard dehydration and embedding. Images were gathered using a JEOL 4000, magnification of 15000.0, accelerating voltage, 400.0 kV.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
regular cardiac myocyte
Cellular Component
intercalated disc
Masahiko Hoshijima
Takeharu Hayashi
CCDB 3603
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
Parameters Imaged
electron density
Visualization Methods
uranyl salt
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——