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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40008*  Cite 

An image from the slice by slice reconstruction of a double tilt series of tomograms of a Caulobacter Crescentus specimen. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous acetone. High Pressure Freezing (HPF) using Balzers HPM 010 in 100 micron deep gold plated copper planchettes. Bacteria was centrifuged decanted and paced applied to planchettes. Freeze-substitution was conducted using this time and temperature schedule: -90 Celsius for 72 hours, raised 2 Celsius per hour for 15 hours, held at -60 Celsius for 12 hours, raised 5 Celsius per hour for 12 hours, held at -20 Celsius. Film images were acquired using a JEOL 4000, at a magnification of 30,000X, 400.0 Kv.

Biological Sources
NCBI Organism Classification
Caulobacter crescentus CB15
Lucy Shapiro
Harley McAdams
CCDB 3628
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
uranyl salt
Processing History
recorded image
Data Qualifiers
processed data
Sample Preparation
osmium tetroxide fixed tissue
cryofixed tissue
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——