Summed Z projection through a through-focus series of a medium spiny neuron from the neostriatum of a dopamine-transporter knock out mouse, filled with Lucifer Yellow and then photooxidized. The neuron is not completely contained within the section so part of it is missing. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database, where the reconstruction of the entire cell can also be found.
Tissue was prepared from a 6 month adult male mouse strain, C57BL/129SvJ with a dopamine transporter knock-ou. Tissue sections, 100µm, were generated using a vibratome. The entire through series of images were gathered using a Bio-Rad 1024 confocal with a Zeiss Plan Apochromat 63X 1.4 NA.