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Licensing
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40024*  Cite 
Description

A maximum intensity projection image of a caulobacter crescentus specimen taken on an IVEM.

Technical Details

Caulobacter cultures (strain Snc04) induced for 1 hour at 30 degrees C, labeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconverted. Samples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide/potassium ferrocyanide post-fixation, dehydration and embedding. Film images were acquired using a JEOL 4000, at a magnification of 30,000X.

Biological Sources
NCBI Organism Classification
Caulobacter crescentus CB15
Attribution
Names
Lucy Shapiro
Harley McAdams
Link
CCDB 3699
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40024
Archival Resource Key (ARK)
ark:/b7295/w9cil40024
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
uranyl salt
Processing History
recorded image
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——