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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40217*  Cite 

Wide field fluorescent image of cultured neuroblastoma cells labeled with phalloidn FITC (green) and DAPI (blue) nuclear stain. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For further information, see Weimiao Yu, Hwee Kuan Lee, Srivats Hariharan, Wen Yu Bu and Sohail Ahmed. Evolving Generalized Voronoi Diagrams of Active Contours for Accurate Cellular Image Segmentation. Cytometry Part A 77A pg. 379~386, 2010.

Technical Details

NIE115 neuroblastoma cells were grown on 18 X 18 mm lamin coated glass coverslips maintained at 37 deg C in 5% CO2. Culture medium was Dulbecoo's Modified Eagle's medium supllemented with 4500 mg/ml glucose and 1% penicillin-streptomycin as an antibiotic. Cells were serum starved for 48 hrs and then fixed with 4% paraformaldehyde, then counterstained with DAPI and FITC-phalloidin. Images were acquired with a Zeiss epifluorescence microscope and a 20X objective.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cell Line
Cellular Component
cell body
actin cytoskeleton
Weimiao Yu
Hwee Kuan Lee
Srivats Hariharan
Wen Yu Bu
Sohail Ahmed
CCDB 6843
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
4',6-diamidino-2-phenylindole (DAPI)
Fluorescein (FITC)
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px ——
Y 382px ——