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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40405*  Cite 
Description

Rendered 3D projection of a confocal z-series taken of rhizome (underground portion of the stem) of Convallari majalis (Lily of the Valley). The green objects represent reconstructed chloroplasts; cell walls are shown in red.

Technical Details

Images were acquired with the Leica TCS AOBS SP2 confocal microscopy using a 63x oil immersion objective (NA 1.40) and an argon/krypton ion laser at wavelengths of 488 nm and an helium/neon 543 nm laser. The fluorescence signals were detected at 500-541 nm and 604-674 nm respectively. Z-series profiles of 30 optical sections were collected at intervals of 1.42 micrometers with a line average of 4 to reduce noise. Maximum projection of the series was applied. After confocal acquisition, z-stack was analysed with IMARIS software to adjust colours and to create 3D renders of chloroplasts.

Biological Sources
NCBI Organism Classification
Convallaria majalis
Cellular Component
chloroplast
cell wall
Biological Context
Biological Process
chloroplast localization
organelle organization
Plant Growth
rhizome
Attribution
Names
Marta Guervos
Link
Andor Insight Awards
Andor Technology
Bitplane
OTHER
Image Processing Unit. University of Oviedo, Spain
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40405
Archival Resource Key (ARK)
ark:/b7295/w9cil40405
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Processing History
3D volume render
Sample Preparation
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1349px ——
Y 1138px ——