Licensing

This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40593^* Cite

Description

Colorized scanning electron micrograph of methicillin resistant staphylococcus aureus (MRSA) bacteria on the surface of a wound dressing.

Technical Details

Specimen was fixed with glutaraldehyde and dehydrated through an ethanol series. It was coated with 5nm gold-palladium and imaged at ultra high resolution (semi-immersion optics). The image was collected on a FEI Nova NanoSEM Family with the following imaging parameters: Magnification: 18,501 x, Horizontal Field Width: 16.1 microns, Vacuum: High vacuum (approx 10 e-06 Torr), Voltage: 5kV, Detector: TLD, Spot: 1.5 nA, and Working Distance: 4.3 mm

Biological Sources

NCBI Organism Classification: Staphylococcus aureus
Cellular Component: cell surface

Biological Context

Biological Process

Attribution

Names: Paul Gunning
Link: FEI Life Sciences Images

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40593
Archival Resource Key (ARK): ark:/b7295/w9cil40593

Imaging

Image Type: recorded image
Image Mode: scanning electron microscopy (SEM)
Parameters Imaged: elastic scattering of electrons
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: visualization of contiguous regions; shadowing and plating; gold; palladium
Processing History: colorized

Sample Preparation

Methods: glutaraldehyde fixed tissue; ethanol dehydration

Dimensions

Spatial Axis	Image Size	Pixel Size
X	670px	——
Y	617px	——