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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40664*  Cite 
Description

Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Yellow boxes are enlarged in panels with corresponding numbers. Box 4, interaction of actin filaments (cyan) with an axonal microtubule (red). Box 5, branched actin filaments (cyan) in the neck of respective spines. This image is part of a group of images representing Figure 2B and its components from Mol Biol Cell. 2010 Jan 1;21(1):165-76. Figure 2B (this image) appears as CIL 40664. The uncolorized overview panel is CIL 40667. Box 4 appears as CIL 40665, and Box 5 appears as CIL 40666.

Technical Details

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
hippocampal neuron
Cellular Component
dendritic spine
dendritic shaft
actin cytoskeleton
microtubule cytoskeleton
Biological Context
Biological Process
dendritic spine organization
dendrite morphogenesis
Attribution
Names
Farida Korobova
Tatyana Svitkina
Published
Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4.
Pubmed
19889835
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40664
Archival Resource Key (ARK)
ark:/b7295/w9cil40664
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Processing History
colorized
Dimensions
Spatial Axis Image Size Pixel Size
X 981px ——
Y 2586px ——