Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40668^* Cite

Description

Time lapse movie prepared from spinning disc confocal images of Drosophila S2 cells stably expressing pMT-Eos2-alpha tubulin. A diagonal ~2.5 micrometer strip was photoconverted and images taken every minute for 20 minutes. Microtubules clearly migrate beyond the confines of the photoconverted strip. This movie is part of a grouped set that includes the corresponding time series stack of images.

Technical Details

Time series were recorded using a Nikon U-2000 inverted microscope equipped with a Nikon 100x NA 1.4 objective lens, Perfect Focus System, CSU-10 spinning disc confocal head (Yokogawa) and Evolve EMCCD camera (Roper Scientific) driven by Nikon Elements software. See also: A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster
Cell Line: S2
Cellular Component: microtubule; cytoskeleton

Biological Context

Biological Process: microtubule cytoskeleton organization; microtubule

Attribution

Names: Vladimir Gelfand; Urko del Castillo

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40668
Archival Resource Key (ARK): ark:/b7295/w9cil40668

Grouping This image is part of a group.

Imaging

Image Type: electron multiplying CCD (EMCCD)
Image Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: mEOS2FP

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	411px	——
Y	456px	——

Time	60 seconds	20