Licensing

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41672^* Cite

Description

Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA001619 (shown in green). HPA001619 is a polyclonal anti-EIF4ENIF1 antibody that labels vesicles. The cells are also stained with a reference marker in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probe was used as reference; DAPI for the nucleus (blue).

Technical Details

The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epidermoid carcinoma
Cell Line: A-431
Cellular Component: vesicle; nucleus

Biological Context

Biological Process: organelle organization; cytoskeleton organization

Attribution

Names: M Uhlen; P Oksvold; L Fagerberg; E Lundberg; K Jonasson; M Forsberg; M Zwahlen; C Kampf; K Wester; S Hober; H Wernerus; L Björling; F Ponten
Published: Nat Biotechnol. 2010 28(12):1248-50.
Pubmed: 21139605
Link: Human Protein Atlas

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41672
Archival Resource Key (ARK): ark:/b7295/w9cil41672

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: primary antibody plus labeled secondary antibody

Sample Preparation

Methods: chemically fixed tissue; permeabilized tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1254px	——
Y	1191px	——