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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41741*  Cite 

Transmission electron micrograph of an unstained, unfixed cytochrome c oxidase crystal prepared from beef heart mitochondria imaged in the frozen hydrated state. A crystalline organization is seen within the centered ovoid structure.

Technical Details

Samples of cytochrome c oxidase in suspension were applied to holey carbon films in a water saturated atmosphere, blotted, and plunged into a cryogen consisting of a mixture of 95% propane and 5% isopentane cooled by liquid nitrogen. Grids were examined using a Philips 420T TEM and images recorded at 46KX in the frozen hydrated state on Kodak SO-163 film. Negatives were scanned with a pixel size of 20x20 micrometers. See also: Frey TG and Murray JM 1994. Electron microscopy of cytochrome c oxidase crystals. J Mol Biol 237:275-297.

Biological Sources
NCBI Organism Classification
Bos sp.
Cell Type
Cellular Component
Cytochrome c oxidase
Biological Context
Biological Process
aerobic respiration
Molecular Function
electron transfer
John Murray
Terence Frey
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
Visualization Methods
visualization of contiguous regions
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
cryofixed tissue
Relation To Intact Cell
isolated subcellular component
Spatial Axis Image Size Pixel Size
X 3782px 0.43nm
Y 4636px 0.43nm