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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:42157*  Cite 
Description

Time lapse movie of P-cadherin +/- epithelium (mammary organoid) branching normally in 3D collagen I matrix. The movie shows minimal cell dissemination and retention of complete myoepithelial coverage. The cells are expressing td tomato as a membrane marker and eEGFP-actin using a keratin-14 promoter. Images were collected every 20 minutes. Image size is 350 x 350 microns. A movie of a normal mouse mammary organoid in collagen I can be found at CIL 42155. This movie is part of a group of movies that include CIL 42151-42168.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
epithelial cell
Cellular Component
plasma membrane
actin cytoskeleton
Biological Context
Biological Process
dissemination
Mouse Gross Anatomy
mammary organoid
Attribution
Published
PNAS 2012 ; published ahead of print August 23, 2012
OTHER
Andrew Ewald
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL42157
Archival Resource Key (ARK)
ark:/b7295/w9cil42157
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
tdTomatoFP
EGFP
Processing History
contrast adjusted
confocal stack
Sample Preparation
Methods
living tissue
Relation To Intact Cell
mammary organoid
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——