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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:45764*  Cite 
Description

Plate 20630 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules. Images The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay). This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

Technical Details

Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery. Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities. The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
U-2 OS
Cellular Component
cell
Biological Context
Biological Process
response to chemical stimulus
Attribution
Names
Sigrun Gustafsdottir
Vebjorn Ljosa
Katherine Sokolnicki
Joshua Bittker
Nicole Bodycombe
Mark Bray
Vlado Dancik
Tom Hasaka
Suk Hon
Melissa Kemp
Kejie Li
Deepika Walpita
Mathias Wawer
Todd Golub
Stuart Schreiber
Paul Clemons
Anne Carpenter
Alykhan Shamji
Link
Broad Institute
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL45764
Archival Resource Key (ARK)
ark:/b7295/w9cil45764
Compounds
11a-ACETOXYPROGESTERONE
1,2alpha-EPOXY-7-DEACETOXY-7-OXODIHYDROGEDUNIN
1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX]
1-PHENYLBIGUANIDE HYDROCHLORIDE
1S,9R-HYDRASTINE
2,4-DICHLOROPHENOXYACETIC ACID
2,6-DI-t-BUTYL-4-METHYLPHENOL
2-ISOPROPYL-3-METHOXYCINNAMIC ACID
2-MERCAPTOBENZOTHIAZOLE
2'-METHOXYFORMONETIN
2-PROPYL-3-HYDROXYETHYLENEPYRAN-4-ONE
3,7-DIHYDROXYFLAVONE
3alpha-ACETOXYDIHYDRODEOXYGEDUNIN
3-AMINO-beta-PINENE
3-HYDROXYBENZYLHYDRAZINE DIHYDROCHLORIDE
3-HYDROXYCOUMARIN
3-PRENYL-4-HYDROXYACETOPHENONE
4,7-DIMETHOXYFLAVONE
4'-DEMETHYLEPIPODOPHYLLOTOXIN
4-METHYLDAPHNETIN
4-NAPHTHALIMIDOBUTYRIC ACID
5,2'-DIMETHOXYFLAVONE
5,7-DIMETHOXYISOFLAVONE
5-FLUOROINDOLE-2-CARBOXYLIC ACID
6,4'-DIMETHOXYFLAVONE
7,2'-DIMETHOXYFLAVONE
7-[2-TRIFLUOROMETHYL-4-(2-HYDROXYPHENYL)-1,3-DIOXAN-cis-5-YL]-HEPT-5Z-ENOIC ACID
7-DESACETOXY-6,7-DEHYDROGEDUNIN
7-HYDROXY-2'-METHOXYISOFLAVONE
7-HYDROXY-8,4'-DIMETHOXYISOFLAVONE
8beta-HYDROXYCARAPIN
8-CYCLOPENTYLTHEOPHYLLINE
8-IODOCATECHIN TETRAMETHYL ETHER
ACECAINIDE HYDROCHLORIDE
ACECLIDINE HYDROCHLORIDE
ACETOCHLOR
ACETOSYRINGONE
ACETYLTRYPTOPHANAMIDE
ALCLOMETAZONE DIPROPIONATE
ALDICARB
ALFLUZOCIN
ALMOTRIPTAN
alpha-CYANO-4-HYDROXYCINNAMIC ACID
ALPINETIN METHYL ETHER
AMCINONIDE
AMINACRINE
AMLODIPINE BESYLATE
ANABASAMINE HYDROCHLORIDE
ANGOLENSIN (R)
ANHYDROBRAZILIC ACID
ANISODAMINE
ANTIAROL
APIIN
ASARYLALDEHYDE
ATENOLOL
ATRAZINE
AUSTRICINE
BENTAZON
BENZO[a]PYRENE
BENZYLBUTYLPHTHALATE
BERGAPTEN
BERGAPTOL
BERGENIN
BETAHISTINE HYDROCHLORIDE
BETAMETHASONE 17,21-DIPROPIONATE
BETAMIPRON
BIOCHANIN A
BROMHEXINE HYDROCHLORIDE
CANRENOIC ACID, POTASSIUM SALT
CARVEDILOL TARTRATE
CEDRELONE
CEFDITORIN PIVOXIL
CHELIDONINE (+)
CHLOROXYLENOL
CHLORPROPHAM
CHROMOCARB
CINNARAZINE
CITROPTEN
CLOBETASOL PROPIONATE
CLOMIPHENE CITRATE
CLOPAMIDE
CORTISONE
COSMOSIIN HEXAACETATE
COUMARIN
COUMOPHOS
CRINAMINE
CRUSTECDYSONE
CURCUMIN
CYCLIZINE
CYCLOPIAZONIC ACID
CYTISINE
DACTINOMYCIN
DANTRON
DAPHNETIN
DEACETOXY-7-OXISOGEDUNIN
DEHYDROROTENONE
DEOXODEOXYDIHYDROGEDUNIN
DEOXYANDIROBIN
DEOXYGEDUNOL ACETATE
DEOXYSAPPANONE B TRIMETHYL ETHER
DERRUBONE
DERRUSTONE
DESACETYL (7)KHIVORINIC ACID, METHYL ESTER
DESOXYPEGANINE HYDROCHLORIDE
DIAZEPAM
DIBUCAINE HYDROCHLORIDE
DIBUTYL PHTHALATE
DICHLOBENIL
DICHLORODIPHENYLTRICHLOROETHANE
DIFFRACTIC ACID
DIFUCOL HEXAMETHYL ETHER
DIHYDRO-beta-TUBAIC ACID
DIHYDROERGOTAMINE MESYLATE
DILTIAZEM HYDROCHLORIDE
DIMETHOATE
DIMETHYL 4,4-o-PHENYLENE-BIS (3-THIOPHANATE)
DIPYROCETYL
DIURON
DUARTIN, DIMETHYL ETHER
EMODIC ACID
ENDECAPHYLLIN X
EPICATECHIN PENTAACETATE
ERGONOVINE MALEATE
ESCITALOPRAM OXALATE
ESTRADIOL-3-SULFATE, SODIUM SALT
ESTRADIOL BENZOATE
ESTRADIOL METHYL ETHER
ESTRONE
ESTRONE BENZOATE
ETHOPROP
ETOPOSIDE
EUGENITOL
EUPARIN
FELAMIDIN
FENAMISAL
FENBUTYRAMIDE
FISSINOLIDE
FLAVOKAWAIN B
FLOPROPIONE
FLUCONAZOLE
FLUNISOLIDE
FLUNIXIN MEGLUMINE
FLURANDRENOLIDE
FORMONONETIN
FUREGRELATE SODIUM
GEMIFLOXACIN MESYLATE
GENISTEIN
GENKWANIN
GINKGETIN, K salt
GITOXIGENIN
GITOXIN
GUANABENZ ACETATE
HAEMATOMMIC ACID
HARMALOL HYDROCHLORIDE
HEXACHLOROPHENE
HUPERZINE A
HYDRALAZINE HYDROCHLORIDE
HYDROXYPROGESTERONE
HYDROXYTOLUIC ACID
ICARIIN
IPRIFLAVONE
IRIFLOPHENONE TRIMETHYL ETHER
IRIGENIN, 7-BENZYL ETHER
IRIGENIN TRIMETHYL ETHER
IRIGENOL
IRILIN A DIMETHYL ETHER
ISOGINKGETIN
ISOLIQUIRITIGENIN
ISOXSUPRINE HYDROCHLORIDE
JUAREZIC ACID
KETOCONAZOLE
KINETIN RIBOSIDE
KOPARIN 2'-METHYL ETHER
LANATOSIDE C
LAPACHOL
LIOTHYRONINE
LIQUIRITIGENIN DIMETHYL ETHER
LOBARIC ACID
LOMATIN
LONCHOCARPIC ACID
LONIDAMINE
LORATADINE
LOSARTAN
MAACKIAIN
MACLUROXANTHONE
MADECASSIC ACID
MEBHYDROLIN NAPHTHALENESULFONATE
MENTHYL BENZOATE
MEPIVACAINE HYDROCHLORIDE
METAMECONINE
METHICILLIN SODIUM
METHIONYL-LEUCYLPHENYLALANINE ACETATE
METHOMYL
METHOXYVONE
METHYL ROBUSTONE
METOLACHLOR
MITOMYCIN C
MODAFINIL
MOLSIDOMINE
MUNINGIN
N- (2-AMINOETHYL)-4-CHLOROBENZAMIDE (RO-16-6491)
N- (9-FLUORENYLMETHOXYCARBONYL)-L-LEUCINE
NAFCILLIN SODIUM
NALOXONE HYDROCHLORIDE
NARINGENIN
NARINGIN
NEOHESPERIDIN DIHYDROCHALCONE
N-FORMYLMETHIONYL-LEUCYLPHENYLALANINE
NICOTINE DITARTRATE
NILOTICIN
N-METHYL (-)EPHEDRINE
NORHARMAN
NORSTICTIC ACID
NOVOBIOCIN SODIUM
OBACUNOL
ONONETIN
ORSELLINIC ACID, ETHYL ESTER
ORTHOTHYMOTINIC ACID
OSAJIN
OSELTAMIVIR TARTRATE
OUABAIN
OXACILLIN SODIUM
OXIBENDAZOLE
PACHYRRHIZIN
PAEONOL
PANTOPRAZOLE
PARAXANTHINE
PARTHENOLIDE
PECTOLINARIN
PENTACHLOROPHENOL
PERICIAZINE
PERINDOPRIL ERBUMINE
PHENACEMIDE
PHENOLPHTHALEIN
PHENOTHRIN
PHENYLBUTYRIC ACID
PHYSOSTIGMINE SALICYLATE
PICEID
PIPERONYLIC ACID
PLUMBAGIN
PONALRESTAT
PREDNISOLONE
PREDNISOLONE ACETATE
PREDNISONE
PRENYLETIN
PRIEURANIN ACETATE
PROCATEROL HYDROCHLORIDE
PROMETON
PROMETRYN
PROPANIL
PROPAZINE
PROPENTOFYLLINE
PRUNETIN
PSEUDOEPHEDRINE HYDROCHLORIDE
PUROMYCIN HYDROCHLORIDE
PURPUROGALLIN
QUIPAZINE MALEATE
RACEPHEDRINE HYDROCHLORIDE
RAMELTEON
RHETSININE
RHOIFOLIN
RITODRINE HYDROCHLORIDE
ROBUSTIC ACID
ROBUSTONE
ROFECOXIB
SALICYL ALCOHOL
SALICYLAMIDE
SALSOLIDINE
SALSOLINE
SELAMECTIN
SERICETIN DIACETATE
SERTRALINE HYDROCHLORIDE
SILIBININ
SIMAZINE
SINOMENINE
SITAGLIPTIN
SOLIDAGENONE
SULFANITRAN
SULPIRIDE
SUPROFEN METHYL ESTER
SYRINGIC ACID
TEBUTHIURON
TELENZEPINE HYDROCHLORIDE
TELMISARTAN
TESTOSTERONE
TETRAC
TETRAHYDROTRIMETHYLHISPIDIN
THEOPHYLLINE
THIAMYLAL SODIUM
THONZYLAMINE HYDROCHLORIDE
TIMOLOL MALEATE
TOLMETIN SODIUM
TOLPERISONE HYDROCHLORIDE
TOTAROL
TRANDOLAPRIL
TRANYLCYPROMINE SULFATE
TRIACETYLRESVERATROL
TRIALLATE
TRIAMCINOLONE ACETONIDE
TRIPELENNAMINE CITRATE
TROXERUTIN
TUBAIC ACID
TULOBUTEROL
VALACYCLOVIR HYDROCHLORIDE
VALSARTAN
VARDENAFIL HYDROCHLORIDE
VESAMICOL HYDROCHLORIDE
VULPINIC ACID
XANTHOXYLIN
YOHIMBIC ACID
YOHIMBINE HYDROCHLORIDE
ZOLPIDEM
ZOMEPIRAC SODIUM