Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:45896*  Cite 
Description

Plate 25674 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules. Images The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay). This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

Technical Details

Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery. Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities. The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
U-2 OS
Cellular Component
cell
Biological Context
Biological Process
response to chemical stimulus
Attribution
Names
Sigrun Gustafsdottir
Vebjorn Ljosa
Katherine Sokolnicki
Joshua Bittker
Nicole Bodycombe
Mark Bray
Vlado Dancik
Tom Hasaka
Suk Hon
Melissa Kemp
Kejie Li
Deepika Walpita
Mathias Wawer
Todd Golub
Stuart Schreiber
Paul Clemons
Anne Carpenter
Alykhan Shamji
Link
Broad Institute
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL45896
Archival Resource Key (ARK)
ark:/b7295/w9cil45896
Compounds
1,3-benzothiazol-2-yl(1,4-thiazinan-4-yl)methanone
BRD-A01283587
BRD-A02126215
BRD-A05083276
BRD-A05907206
BRD-A06221803
BRD-A09409207
BRD-A13753515
BRD-A15615781
BRD-A17724553
BRD-A17970083
BRD-A19908948
BRD-A21346136
BRD-A25056678
BRD-A26616161
BRD-A27546103
BRD-A27762595
BRD-A30356435
BRD-A30570056
BRD-A31650268
BRD-A32325811
BRD-A33095017
BRD-A33642205
BRD-A34384930
BRD-A35506209
BRD-A40336966
BRD-A44748053
BRD-A45657137
BRD-A45839063
BRD-A45997690
BRD-A49197143
BRD-A54878426
BRD-A54950948
BRD-A55734193
BRD-A59804189
BRD-A62606688
BRD-A62750882
BRD-A63464390
BRD-A71097331
BRD-A72713024
BRD-A73802569
BRD-A74927609
BRD-A75439785
BRD-A79846938
BRD-A82602065
BRD-A85713941
BRD-A87909953
BRD-A89697488
BRD-A92289882
BRD-A93998311
BRD-A96155639
BRD-A96470865
BRD-A96824985
BRD-A98886817
BRD-K00052649
BRD-K00663527
BRD-K00891298
BRD-K01025623
BRD-K01149656
BRD-K02057599
BRD-K02247990
BRD-K02387605
BRD-K02581454
BRD-K02628096
BRD-K02648154
BRD-K02671913
BRD-K03373655
BRD-K03795998
BRD-K03750483
BRD-K04262424
BRD-K04667280
BRD-K04847028
BRD-K04905134
BRD-K04948770
BRD-K05011543
BRD-K05105053
BRD-K05835964
BRD-K06110588
BRD-K06329530
BRD-K06442596
BRD-K07509832
BRD-K07689019
BRD-K08295340
BRD-K08309090
BRD-K08604406
BRD-K08836529
BRD-K09018805
BRD-K09287266
BRD-K09411557
BRD-K09718177
BRD-K09906118
BRD-K11219527
BRD-K11233651
BRD-K11533282
BRD-K11875614
BRD-K12535899
BRD-K12745086
BRD-K12782798
BRD-K12837934
BRD-K13111496
BRD-K14856054
BRD-K16568308
BRD-K16737809
BRD-K18074447
BRD-K18353242
BRD-K18700174
BRD-K18915563
BRD-K19137242
BRD-K19576518
BRD-K19668300
BRD-K21283872
BRD-K21686355
BRD-K21693855
BRD-K22293207
BRD-K22397749
BRD-K22541765
BRD-K22844799
BRD-K24253116
BRD-K24355197
BRD-K25945985
BRD-K26244720
BRD-K26303018
BRD-K28740088
BRD-K29697209
BRD-K29955010
BRD-K30049194
BRD-K31040683
BRD-K31169178
BRD-K31350933
BRD-K31469986
BRD-K32577024
BRD-K32930883
BRD-K33742819
BRD-K33855136
BRD-K34009558
BRD-K34333932
BRD-K34526507
BRD-K34885859
BRD-K34901837
BRD-K35038394
BRD-K35066423
BRD-K35601142
BRD-K35666708
BRD-K36551994
BRD-K37794655
BRD-K39458989
BRD-K39597613
BRD-K39796608
BRD-K39875859
BRD-K39927438
BRD-K39939258
BRD-K40636143
BRD-K41024230
BRD-K41727577
BRD-K42379819
BRD-K42388113
BRD-K42634295
BRD-K43184094
BRD-K43454581
BRD-K43684360
BRD-K43909184
BRD-K44122401
BRD-K44146005
BRD-K44849349
BRD-K44973649
BRD-K45171984
BRD-K45418850
BRD-K45835784
BRD-K46341768
BRD-K46369268
BRD-K46432246
BRD-K46758233
BRD-K46915789
BRD-K46982937
BRD-K47119189
BRD-K47863178
BRD-K47933790
BRD-K48125437
BRD-K48576794
BRD-K48696144
BRD-K48788686
BRD-K49394549
BRD-K49683533
BRD-K49713996
BRD-K50229096
BRD-K51215204
BRD-K51219376
BRD-K52033610
BRD-K52259918
BRD-K53014614
BRD-K53088149
BRD-K53315710
BRD-K53418960
BRD-K54265631
BRD-K54453195
BRD-K54566838
BRD-K54842031
BRD-K55131074
BRD-K55621929
BRD-K56679803
BRD-K56872287
BRD-K56947701
BRD-K58455347
BRD-K58462572
BRD-K58481857
BRD-K58486677
BRD-K58679021
BRD-K58985014
BRD-K59799058
BRD-K60163230
BRD-K61456580
BRD-K61780473
BRD-K61802148
BRD-K61947206
BRD-K62878219
BRD-K63088676
BRD-K63259048
BRD-K63298572
BRD-K63586469
BRD-K63632273
BRD-K64182643
BRD-K64472183
BRD-K64531535
BRD-K65118526
BRD-K65130752
BRD-K65489619
BRD-K65532249
BRD-K65584944
BRD-K65889891
BRD-K65985499
BRD-K66650250
BRD-K66655179
BRD-K66708529
BRD-K66837299
BRD-K67122145
BRD-K67619511
BRD-K67882163
BRD-K69556030
BRD-K70512109
BRD-K70698180
BRD-K70831943
BRD-K70982665
BRD-K71600228
BRD-K71688948
BRD-K72294294
BRD-K72469029
BRD-K72684508
BRD-K73883424
BRD-K74009522
BRD-K74016717
BRD-K74480682
BRD-K75175199
BRD-K75728245
BRD-K75874680
BRD-K76023535
BRD-K76203848
BRD-K76210886
BRD-K76506856
BRD-K78443863
BRD-K78599916
BRD-K79246618
BRD-K79252316
BRD-K80516501
BRD-K80727510
BRD-K81254093
BRD-K82484613
BRD-K82641815
BRD-K83061778
BRD-K83251175
BRD-K83271024
BRD-K83897129
BRD-K84108405
BRD-K84115730
BRD-K84447645
BRD-K85027801
BRD-K85779937
BRD-K85900774
BRD-K86168888
BRD-K87005324
BRD-K87163849
BRD-K87657228
BRD-K87805974
BRD-K88272724
BRD-K88734556
BRD-K90676269
BRD-K91137263
BRD-K91188267
BRD-K91229509
BRD-K91660991
BRD-K91912994
BRD-K92005363
BRD-K92291012
BRD-K92378369
BRD-K92944586
BRD-K93254057
BRD-K93651844
BRD-K93659802
BRD-K93702178
BRD-K94342340
BRD-K94469430
BRD-K96052120
BRD-K96069363
BRD-K96447304
BRD-K96711684
BRD-K97249368
BRD-K97455960
BRD-K97532930
BRD-K97558524
BRD-K97931214
BRD-K98146210
BRD-K98217168
BRD-K98617141
BRD-K98782887
BRD-K99185324
BRD-K99727583
BRD-K99955175
BRD-K99978353
BRD-K99995088
BRD-M58554218
Concentramide