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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:4662*  Cite 
Description

High resolution of a daughter micronucleus in anaphase of a dividing cell of Didinium nasutum. The chromosomes are compacted into one large heterochromatic body. Remnants of the mitotic division spindle remain inside the intact nuclear envelope. Nuclear envelopes remain intact in ciliates during division in both the micronucleus and macronucleus. Mitochondrion, peroxisome, dictyosomes of Golgi complex, and rough endoplasmic reticulum are evident. TEM taken on 5/19/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with a Nikon Coolscan 9000ED. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Didinium nasutum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Company, NC, U.S.A.
Cellular Component
micronucleus
nuclear heterochromatin
condensed nuclear chromosome
nuclear envelope
spindle microtubule
Biological Context
Biological Process
mitotic anaphase
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL4662
Archival Resource Key (ARK)
ark:/b7295/w9cil4662
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 5582px 1nm
Y 3679px 1nm