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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:502*  Cite 
Description

Echinoderm sperm nuclei are unusual in that chromatin fibers are clearly seen, allowing their diameter to be accurately determined. Here, sperm from the starfish P. miniata were treated to remove the outer membrane, cryo-fixed by plunging into liquid ethane, freeze-substituted in acetone and low-temperature embedded in Lowicryl K11M. Thin sections were stained with uranyl acetate, and examined using a FEI CM10 TEM operated at 100KV. This image shows the cup-shaped nucleus surrounding the acrosome. Flagellar axonemes are also present. Chromatin fibers in the nucleus are clearly visualized.

Biological Sources
NCBI Organism Classification
Patiria miniata
Cell Type
sperm
Cellular Component
male germ cell nucleus
Biological Context
Biological Process
reproduction
nucleus organization
Molecular Function
structural constituent of chromatin
Attribution
Names
Chris Woodcock
Pubmed
8138565
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL502
Archival Resource Key (ARK)
ark:/b7295/w9cil502
Grouping This image is part of a group.
Imaging
Image Type
recorded image
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
Methods
cryofixed tissue
freeze substitution
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 4160px 1.2nm
Y 2816px 1.2nm