Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50302^* Cite

Description

Transmission electron micrograph of a portion of a type II alveolar cell from an aged guinea pig showing unusual protein within the RER.

Technical Details

Species: cobaya
Gender: male
Weight: 250-500g
Subspecies or gene: Hartley white short hair air and vitamin C lung type II cell intracisternal bodies, ribosomes, lamellar bodies
Organ: mid ventral right lobe lung
Tissue: lung
Component: unusual proteins, probably surfactant proteins within the RER
Grouped: intracisternal protein
Accelerating voltage: 60kv
Stain 1: 1% osmium tetroxide
Stain 2: lead citrate and uranyl acetate
Embed: Spurr's resin or epon 812
Section: 50-100 nm thick

Biological Sources

NCBI Organism Classification: Cavia
Cell Type: alveolar type II cell

Attribution

Names: Marian Miller; Brooks Adams

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL50302
Archival Resource Key (ARK): ark:/b7295/w9cil50302

Imaging

Image Type: micrograph
Image Mode: transmission electron microscopy (TEM)

Sample Preparation

Methods: Glutaraldehyde and paraformaldehyde in phosphate buffer

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4000px	——
Y	4517px	——