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Bacteria imaged inside the intestines of live zebrafish (Danio rerio) larvae, 5-6 days post-fertilization. The bacteria were engineered to constitutively express Green Fluorescent Protein (GFP), or dTomato for one dataset as indicated in the README.txt file; genetic techniques are described in T. J. Wiles et al. "Modernized tools for streamlined genetic manipulation of wild and diverse symbiotic bacteria." mBio, 9: e01877-18 (2018).

Technical Details

All images are 28x28x8 pixel (4.5x4.5x8 microns) subsets of three-dimensional images spanning entire larval zebrafish intestines. xy-scale: 0.1625 microns/pixel; z-scale: 1 micron/slice.

Light sheet fluorescence microscope, home-built, Zeiss Plan Apochromat, 40x/1.0 NA detection objective. Camera: Cooke pco.edge. The instrument is described in: Taormina MJ, Jemielita M, Stephens WZ, Burns AR, Troll JV, Parthasarathy R, et al. "Investigating Bacterial-Animal Symbioses with Light Sheet Microscopy". The Biological Bulletin. 2012:223(1):7-20. doi:10.1086/BBLv223n1p7 and Ryan Baker, Michael J. Taormina, Matthew Jemielita, and Raghuveer Parthasarathy, "A combined light sheet fluorescence and differential interference contrast microscope for live imaging of multicellular specimens", J. Microscopy 258:105-112 (2015). DOI: 10.1111/jmi.12220.

Biological Sources
Cell Type
Biological Context
Biological Process
Individual bacteria inside larval zebrafish intestine
Edouard Hay
Image Mode
modes of light microscopy