Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:700^* Cite

Description

X-ray scattering shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contain more ordered chromatin than most nuclei. Here, fibers isolated from these nuclei have been negatively stained with aurothioglucose and imaged by TEM. ~30 nm chromatin fibers are seen, some exhibiting short regions with apparent structural order.

Technical Details

Chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films, negatively stained with aurothioglucose and observed at 80Kv using a CM10 TEM. See Woodcock CL et al. 1991. Ultrastructure of chromatin. I. Negative staining of isolated fibers. J Cell Sci. 99:99-106.

Biological Sources

NCBI Organism Classification: Necturus maculosus
Cell Type: nucleate erythrocyte
Cellular Component: nuclear chromatin

Biological Context

Biological Process: chromatin assembly; chromosome organization
Molecular Function: DNA packaging

Attribution

Names: Chris Woodcock

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL700
Archival Resource Key (ARK): ark:/b7295/w9cil700

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: other negative stain
Processing History: raw unprocessed data

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2334px	0.67nm
Y	1932px	0.67nm