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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7421*  Cite 
Description

The protein MeCP2 binds to symmetrically methylated CpG dinucleotides and is thought to transmit the (generally) repressive signals encoded in DNA methylation patterns. Mutations in MeCP2 lead to Rett syndrome. Here, MeCP2-DNA complexes have been imaged by AFM (Digital Instruments Nanoscope) in air using human MeCP2 and an 828 bp DNA sequence containing several methylated CpGs. MeCP2, which appears as bright circles in AFM images, and volume measurements of the complexes suggest that MeCP2 monomers and dimers are present and that MeCP2 binds DNA cooperatively. Other images in the linked set show looped structures and large complexes containing multiple DNA strands and MeCP2 molecules. These configurations are likely promoted by the multiple independent DNA-binding domains of MeCP2.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
neuron
Cellular Component
nucleus
Biological Context
Biological Process
transcription regulation
Molecular Function
GO:0016565
Human Disease
Rett syndrome
Attribution
Names
Shlyaktenko, Luda; Woodcock, Chris
Pubmed
20679481
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7421
Archival Resource Key (ARK)
ark:/b7295/w9cil7421
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
microscopy
atomic force microscopy
Parameters Imaged
specimen height
Source of Contrast
specimen height
Processing History
Pixel value determine by specimen height
Sample Preparation
Methods
glutaraldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 1.95nm
Y 512px 1.95nm