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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7600*  Cite 
Description

High resolution transmission electron micrograph of Halteria showing both the large macronucleus with nuclear pores, dense heterochromatin bodies and nucleolus, and the small micronucleus.

Technical Details

TEM taken on 3/12/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Halteria grandinella
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Co., NC, U.S.A.
Cellular Component
macronucleus
micronucleus
nucleolus
nuclear pore
nuclear chromatin
Biological Context
Biological Process
macronuclear organization
micronuclear organization
nucleolar organization
nuclear pore distribution
nucleus organization
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7600
Archival Resource Key (ARK)
ark:/b7295/w9cil7600
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4623px 1.1nm
Y 5114px 1.1nm