Alternate header for print version


Licensing
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7756*  Cite 
Description

Disruption of Microtubules with Nocodazol U2OS human osteosarcoma cells were transduced with 'Cellular Lights' Actin-RFP (Actin-red fluorescent protein) and Cellular Lights MAP4-GFP (MAP4-green fluorescent protein) and imaged the next day. Cells were washed in HBSS and incubated with 1 ug/ml of the DNA stain Hoechst 33342 for 10 minutes then washed with HBSS. Nocodazole, a microtubule inhibitor, was applied to the bath at a final concentration of 10 uM. Images were taken every 20 seconds for 30 minutes. In this video, microtubules appear green, actin microfilaments appear red, and the nucleus is stained blue. Note the loss of the microtubular network (green) whilst the actin cytoskeleton (red) remains intact. Cells were imaged on a Deltavision core microscope with a 40X objective, 1.4 NA. Fluorescence was detected using the appropriate filters (DAPI/FITC/TRITC). The time series was deconvolved using standard software. Scale bar = 20 microns.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
permanent cell line cell
Cell Line
U-2 OS
Cellular Component
microtubule cytoskeleton
actin cytoskeleton
nucleus
Biological Context
Biological Process
actin filament depolymerization
Attribution
Names
Nick Dolman
Rob Batchelor
Penny Melquis
Mike O'Grady
George Hanson
OTHER
Invitrogen, Eugene OR
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7756
Archival Resource Key (ARK)
ark:/b7295/w9cil7756
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
differences in adsorption or binding of stain
Visualization Methods
mRFP1
Hoechst 33342
EGFP
Processing History
deconvolution
Sample Preparation
Methods
unfixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 538px 0.2µm
Y 528px 0.2µm
Channel Wavelength
1 DAPI/FITC/TRITCnm
Time 1800 seconds 20s/frame