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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:8079*  Cite 

The movie starts in metaphase and shows sister kinetochore pairs aligned at the metaphase plate and oscillating between the two spindle poles as their respective kinetochore fibers lengthen and shorten. As the cell, mitotic PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), enters anaphase and the sister chromatids separate, sister kinetochores move away from each other as their kinetochore fibers shorten. However, one of the kinetochores lags behind at the cell equator, thus resulting in a chromosome mis-segregation event known as anaphase lagging chromosome. This type of mis-segregation is the consequence of merotelic kinetochore orientation, a type of kinetochore mis-attachment in which a single kinetochore binds microtubules coming from both spindle poles, rather than just one. The compacted DNA of the chromosome arms excludes the background fluorescence so that chromosome arms appear as dark rod-like structures and the movement of the sister chromatid arms away from each other during anaphase can also be followed. Digital images were collected with an Orca ER cooled CCD camera (Hamamatsu Photonics) coupled to a Yokogawa spinning disk confocal CS10 unit (Perkin Elmer Life Sciences Wallac), which was attached to a Nikon TE300 inverted microscope. Illumination was achieved via an Argon/Krypton laser and 488 nm and 568 nm filters in an excitation filter wheel (Sutter Instruments) through a 60 x 1.4 NA Plan-Apochromatic DIC objective lens. Nearly simultaneous fluorescence images were acquired at 488 and 568 nm at a single focal plane every 20-60 seconds. The movie is played at 10 frames per second. Elapsed time is shown in min:sec. Corresponds to video 2 in Curr Biol. 2004 Dec 14;14(23):2149-55.

Biological Sources
NCBI Organism Classification
Potorous tridactylus
Cell Type
epithelial cell
Cell Line
Pt K1 (NBL-3)
Cellular Component
spindle microtubule
Biological Context
Biological Process
attachment of spindle microtubules to kinetochore
Daniela Cimini
Lisa A Cameron
Edward D Salmon
Curr Biol. 2004 Dec 14;14(23):2149-55.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Alexa Fluor 488
labeled primary antibody
Processing History
color combine
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 302px 0.128µm
Y 295px 0.128µm
Channel Wavelength
1 488,568nm
Time 29 seconds