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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:8104*  Cite 

The organization of the actin cytoskeleton in an early Drosophila melanogaster embryo (cycles 9-12) syncytium can be observed by time lapse imaging of embryos expressing a GFP-tagged actin binding protein, moesin. GFP-moesin illuminates actin caps, which form around each centrosome and are located above each nucleus in interphase and prophase. In prophase, pseudocleavage furrows start to ingress from the caps around each nucleus and the forming spindle, which prevents the fusion of adjacent spindles. A description of the capping arrangement can be found in Postner et al., (1992), J Cell Biol., 119:1205. Time lapse 4D spinning disk confocal microscopy images were acquired every 8 seconds and are played at 6 frames/second. The images were acquired with a Nikon microscope (a X40 objective) and a Perkin-Elmer laser spinning disk. The program used to capture, pseudocolor, and process the images was Metamorph.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cellular Component
actin cytoskeleton
Biological Context
Biological Process
Molecular Function
regulation of mitotic spindle organization
Rosalind Silverman-Gavrila
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Data Qualifiers
processed data
Sample Preparation
living tissue
Spatial Axis Image Size Pixel Size
X 230px ——
Y 510px ——
Time 9 seconds