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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:8105*  Cite 
Description

This movie is of a Drosophila melanogaster early embryo (cycle 10-12) expressing a GFP-tagged centrosomal protein called TACC (transforming, acidic, coiled-coil protein). GFP-TACC marks the movement and behavior of centrosomes in Drosophila early embryogenesis. In early embryonic stages, Drosophila embryos divide syncytially, giving rise to the synchronous behavior observed. Centrsosomes divide very early in the cell cycle, in interphase, and then migrate to opposite poles of the nucleus. The cycle repeats in the next interphase. Time lapse 4D spinning disk confocal microscopy images were acquired every 8 seconds and are played at 6 frames/second. The images were acquired with a Nikon microscope (a X40 objective) and a Perkin-Elmer laser spinning disk. The program used to capture, pseudocolor, and process the images was Metamorph.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cellular Component
spindle pole centrosome
Biological Context
Biological Process
mitosis
Molecular Function
mitotic spindle organization
Attribution
Names
Rosalind Silverman-Gavrila
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL8105
Archival Resource Key (ARK)
ark:/b7295/w9cil8105
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
TagGFP
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 500px ——
Y 506px ——
Time 11 seconds