Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9071^* Cite

Description

Murine adipocytes differentiated from 3T3-L1 cells showing intracellular trafficking of GFP-GLUT4 vesicles. GLUT 4 (Glucose Transporter Type 4) is a glucose transport protein that is responsible for insulin-regulated glucose translocation into the cell. Fluorescence TIRFM Image. Leica TIRFM Objective used: 100X 1.46 NA. Images captured with a Hamamatsu EMCCD camera.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: CL:0000450
Cell Line: 3T3L1
Cellular Component: insulin-responsive compartment

Biological Context

Biological Process: vesicle-mediated transport
Molecular Function: glucose transmembrane transporter activity

Attribution

Names: Yu Chen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9071
Archival Resource Key (ARK): ark:/b7295/w9cil9071

Imaging

Image Type: recorded image
Image Mode: TIRF
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	186px	0.1448µm
Y	320px	0.1451µm

Time	0.2 seconds