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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9233*  Cite 
Description

Immunofluorescence image of HeLa cell expressing a fusion protein of GFP-polypyrimidine tract binding protein showing the localization of the perinucleolar compartment (green area) at the periphery of the nucleolus (red region). The nuclear periphery is demarcated by immunolabeling of the nuclear lamina (blue).

Technical Details

See Huang et al. 1997, The Dynamic Organization of the Perinucleolar Compartment in the Cell Nucleus J. Cell Biol 137: 965-974.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
cervical carcinoma
Cell Line
HeLa
Cellular Component
nucleolus
Biological Context
Biological Process
RNA metabolic process
Attribution
Names
Sui Huang
Thomas J. Deerinck
Mark H. Ellisman
David L. Spector
Pubmed
9166399
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9233
Archival Resource Key (ARK)
ark:/b7295/w9cil9233
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
charge coupled device (CCD)
Parameters Imaged
fluorescence microscopy
Source of Contrast
fluorescence emission
Visualization Methods
TexasRed
EGFP
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 672px 40nm
Y 528px 40nm