Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9255^* Cite

Description

A high resolution of an oblique section of the Coleps contractile vacuole pore revealing the supporting microtubules and adjacent fibrous layer. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/4/69 by R. Allen with Philips 300. Neg. 25,100X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: contractile vacuole pore; microtubule associated complex; axonemal dynein complex; plasma membrane; cytoskeleton; actin cytoskeleton

Biological Context

Biological Process: water homeostasis; microtubule cytoskeleton organization; microfibril organization
Molecular Function: control of water regulation

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9255
Archival Resource Key (ARK): ark:/b7295/w9cil9255

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: film; unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4689px	0.8nm
Y	3204px	0.8nm