Alternate header for print version


Image 2D
Display image description
Single transmitted light section from a through focus series through a photoconverted medium spiny cell from the neostriatum of a wild type mouse. Flocculent material surrounding the labeled cell is likely photoconverted mitochondria or non-specific debris produced by the photoconversion procedure.
Full resolution image description
Zip file containing original transmitted light z series in BioRad PIC format and also a version in multi-image tiff format
Animation description
Animation stepping through a through focus series of a photoconverted medium spiny cell from the neostriatum of a wild type mouse. Flocculent material surrounding the labeled cell is likely photoconverted mitochondria or non-specific debris produced by the photoconversion procedure.

Segmentation
Display image description
Manual tracing of dendrites using Neurolucida. Spines were traced but these were difficult to see, so the number may not be accurate.
Segmentation file description
Zip file containing Neurolucida trace file in ascii format (050803d_finaltrace.ASC), along with the output in VRML format. Summary files of measurements generated by Neuroexplorer for each of the parts traced are also included.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3383*  Cite 
Project: P1207
Project name
Correlative microscopic characterization of dendritic spines in a transgenic mouse model of hyperdopaminergia: The dopamine transporter knockout mouse
Description
Multiscale characterization of DAT KO transgenic mouse
Funding agency
NIH
Leader(s)
Diana Price
Collaborator(s)
Aki Laakso
Michele Cyr
Maryann Martone
Naoko Yamada
Andrea Thor
Monica Berlanga
Start date
01-01-2003
End date
unspecified
 
Experiment
Experiment ID
19
Experiment date
04-22-2003
Title
P1207 Experiment 5
Purpose
EMT reconstructions of medium spiny neuron dendrites
Experimenter(s)
Diana Price
Masako Terada
Andrea Thor
Microscopy product
Microscopy product ID
3383
Instrument
Bio-Rad Radiance 2000
Microscopy type
confocal
Product type
THROUGH FOCUS SERIES
Image basename
050803D
Spatial Axis Image Size Pixel Size
X 1024px 0.24 um/pixels
Y 1024px 0.24 um/pixels
Subject
Species
mouse
Scientific name
Mus Musculus
Strain
C57BL/129SvJ
Group by
genetic manipulation
Treatment
NA
Age
7 months
Age class
adult
Tissue section
Anatomical location
neostriatum 050803D&E
Microtome
Vibratome
Tissue product storage
P1207Slidebox1
Thickness
100 µm
Specimen description
Organ
brain
System
central nervous
Tissue
striatum
Map location
View location
Cell type
medium spiny neuron
Atlas coordinate
0, 0, 0,
Imaging parameters
Type
Light microscopy product
Immersion medium
air
Mounting medium
resin
Lens magnification
X
Numerical aperture
0.75
Notes
mmartone
Imaging product type
Type
Through focus
Description
transmitted light z series through photoconverted medium spiny dendride
Z step
.5 microns
Psf file
050803D